| ID | SAVERM_119
| Type | CDS
|
| From | 136846
| nt length | 645
|
| To | 137490
| aa length | 214
|
| Gene |
| pI | 12.27
|
| Definition | putative IS5 family IS1647-like transposase
| M.W (Da) | 18897
|
| Note | IS5 family (136639..137502) Inverted repeat sequence (18/24 bp). Translation will be controlled by frameshift with SAV118. PF13586: Transposase DDE domain
| AseI frg. | A
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | transposon & IS
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO0081 putative insertion element transposase | 258 | 1.0e-89
|
| sam | SAM23877_5733 transposase | 247 | 4.0e-85
|
| scab | SCAB44411 putative transposase | 246 | 2.0e-84
|
| sace | SACE_2562 transposase, IS4 family, | 160 | 4.0e-51
|
| nfar | nfa20570 transposase | 178 | 2.8e-46
|
| cglu | gi|19553088 COG3293:Predicted transposase [Corynebacterium glutamicum] | 65 | 1.7e-12
|
| ecoli | gi|16129795 DNA polymerase III, theta subunit [Escherichia coli K12] | 26 | 1.0e+00
|
*coel, Streptomyces coelicolor A3(2); sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; cglu, Corynebacterium glutamicum; ecoli, Escherichia coli K-12;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| DDE_Tnp_1 | 1 | Transposase DDE domain | 71-193 | 57.2 | 1.6e-15
|
| DDE_Tnp_1_2 | 1 | Transposase DDE domain | 130-207 | 55.3 | 6e-15
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| EDX23754.1 | conserved hypothetical protein [Streptomyces sp. Mg1]
| 769 | 1.3e-101 |
| EDX20388.1 | conserved hypothetical protein [Streptomyces sp. Mg1] >gb
| 765 | 4.9e-101 |
| EFE72107.1 | conserved hypothetical protein [Streptomyces viridosporus ATCC 14...
| 749 | 1.5e-98 |
| EFL19388.1 | transposase [Streptomyces sp. C]
| 742 | 2.2e-97 |
| SFO12956.1 | Transposase [Streptomyces sp. cf124]
| 673 | 1.2e-87 |
|
| 670 | 3.2e-87 |
| WP_197586338.1 | MULTISPECIES: IS5 family transposase [Streptomyces]
| 674 | 2.2e-86 |
| MYS95838.1 | IS5 family transposase [Streptomyces sp. SID5469]
| 674 | 2.3e-86 |
| KOT96463.1 | transposase [Streptomyces sp. NRRL F-4711] >gb
| 661 | 8.4e-86 |
| KOV59331.1 | transposase [Streptomyces sp. MMG1121]
| 659 | 1.6e-85 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.3756 | Seems to have no N-terminal signal seq.
|