| ID | SAVERM_2389
| Type | CDS
|
| From | 2915874
| nt length | 1302
|
| To | 2914573
| aa length | 433
|
| Gene |
| pI | 6.33
|
| Definition | putative RNA methyltransferase
| M.W (Da) | 46762
|
| Note | PF05958: tRNA (Uracil-5-)-methyltransferase
| AseI frg. | M
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA modification
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5901 undefined product | 793 | 0.0e+00
|
| sgr | SGR_1654 putative RNA methyltransferase | 744 | 0.0e+00
|
| sam | SAM23877_5579 putative RNA methyltransferase | 808 | 0.0e+00
|
| scab | SCAB23931 putative RNA methyltransferase | 810 | 0.0e+00
|
| sace | SACE_1814 23S rRNA methyltransferase/RumA | 319 | 2.0e-106
|
| nfar | nfa37440 RNA methyltransferase | 301 | 9.5e-83
|
| mtub | gi|15609826 CONSERVED HYPOTHETICAL ALANINE AND VALINE AND GLYCINE RICH PROTEIN [Mycobacterium tuberculosis H37Rv] | 231 | 9.2e-62
|
| cglu | gi|19553107 COG2265:SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase [Corynebacterium glutamicum] | 178 | 3.8e-46
|
| bsub | BG12826 yefA, yerS; similar to RNA methyltransferase [EC:2.1.1.-] | 145 | 3.4e-36
|
| ecoli | gi|16130692 23S rRNA (uracil-5-)-methyltransferase [Escherichia coli K12] | 111 | 1.1e-25
|
| paer | PA0933 [gene=ygcA] [prot=probable RNA methyltransferase] [comment=PA0933] | 157 | 2.3e-39
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| tRNA_U5-meth_tr | 1 | tRNA (Uracil-5-)-methyltransferase | 353-427 | 58.9 | 3.9e-16
|
| TRAM | 1 | TRAM domain | 2-58 | 48.4 | 6.2e-13
|
| Methyltransf_31 | 1 | Methyltransferase domain | 283-359 | 36.3 | 4.1e-09
|
| MTS | 1 | Methyltransferase small domain | 277-365 | 23.8 | 2.4e-05
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_190172398.1 | class I SAM-dependent RNA methyltransferase [Streptomyces mi...
| 2170 | 0.0e+00 |
| WP_148010920.1 | class I SAM-dependent RNA methyltransferase [Streptomyces sp...
| 2169 | 0.0e+00 |
| WP_097224571.1 | class I SAM-dependent RNA methyltransferase [Streptomyces sp...
| 2166 | 0.0e+00 |
| WP_217566190.1 | class I SAM-dependent RNA methyltransferase [Streptomyces sp...
| 2165 | 0.0e+00 |
| WP_097289250.1 | MULTISPECIES: class I SAM-dependent RNA methyltransferase [u...
| 2163 | 0.0e+00 |
| WP_075030582.1 | class I SAM-dependent RNA methyltransferase [Streptomyces mi...
| 2162 | 0.0e+00 |
| WP_095852168.1 | class I SAM-dependent RNA methyltransferase [Streptomyces sp...
| 2158 | 0.0e+00 |
| WP_067375340.1 | TRAM domain-containing protein [Streptomyces olivochromogene...
| 2156 | 0.0e+00 |
| WP_168496487.1 | class I SAM-dependent RNA methyltransferase [Streptomyces sp...
| 2156 | 0.0e+00 |
| WP_212726811.1 | TRAM domain-containing protein [Streptomyces mirabilis] >gb
| 2153 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.1192 | Seems to have no N-terminal signal seq.
|