| ID | SAVERM_2501
| Type | CDS
|
| From | 3052491
| nt length | 843
|
| To | 3053333
| aa length | 280
|
| Gene | nei1
| pI | 10.78
|
| Definition | putative endonuclease VIII and DNA N-glycosylase with an AP lyase activity
| M.W (Da) | 30936
|
| Note | involved in DNA repair; nick DNA at apurinic/apyrimidinic sites. PF01149: Formamidopyrimidine-DNA glycosylase N-terminal domain
| AseI frg. | Q
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | DNA modification & repair
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5760 DNA glycosylase | 456 | 1.0e-164
|
| sgr | SGR_1761 putative endonuclease VIII | 376 | 1.0e-132
|
| sam | SAM23877_5464 putative endonuclease 8 2 | 445 | 2.0e-160
|
| scab | SCAB25041 putative endonuclease VIII and DNA N-glycosylase with an AP lyase activity | 456 | 2.0e-164
|
| sace | SACE_5875 endonuclease VIII | 249 | 2.0e-83
|
| nfar | nfa9770 endonuclease VIII | 243 | 1.8e-65
|
| mtub | gi|15610433 PROBABLE ENDONUCLEASE VIII NEI [Mycobacterium tuberculosis H37Rv] | 211 | 3.3e-56
|
| cglu | gi|19552074 COG0266:Formamidopyrimidine-DNA glycosylase [Corynebacterium glutamicum] | 191 | 4.3e-50
|
| ecoli | gi|16128689 endonuclease VIII [Escherichia coli K12] | 117 | 1.1e-27
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; ecoli, Escherichia coli K-12;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Fapy_DNA_glyco | 1 | Formamidopyrimidine-DNA glycosylase N-terminal domain | 1-80 | 41.1 | 2.2e-10
|
| H2TH | 1 | Formamidopyrimidine-DNA glycosylase H2TH domain | 124-203 | 36.1 | 4.5e-09
|
BLAST search result (top 10 hits)
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.2120 | Seems to have no N-terminal signal seq.
|