| ID | SAVERM_2546
| Type | CDS
|
| From | 3123031
| nt length | 954
|
| To | 3122078
| aa length | 317
|
| Gene | ribF
| pI | 5.55
|
| Definition | putative riboflavin kinase
| M.W (Da) | 34648
|
| Note | PF06574: FAD synthetase
| AseI frg. | V
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of coenzymes & prosthetic groups
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5711 probable riboflavin kinase (FAD synthetase) | 567 | 0.0e+00
|
| sgr | SGR_1806 putative riboflavin kinase | 553 | 0.0e+00
|
| sam | SAM23877_5433 Riboflavin biosynthesis protein RibF | 572 | 0.0e+00
|
| scab | SCAB25301 putative riboflavin kinase | 576 | 0.0e+00
|
| sace | SACE_5918 riboflavin kinase / FMN adenylyltransferase | 337 | 1.0e-116
|
| nfar | nfa38900 ribF riboflavin kinase | 332 | 2.0e-92
|
| mtub | gi|15609923 hypothetical protein Rv2786c [Mycobacterium tuberculosis H37Rv] | 327 | 6.4e-91
|
| cglu | gi|19553182 COG0196:FAD synthase [Corynebacterium glutamicum] | 258 | 3.4e-70
|
| bsub | BG11495 ribC; riboflavin kinase / FAD synthase [EC:2.7.1.26 2.7.7.2] | 141 | 4.4e-35
|
| ecoli | gi|16128019 hypothetical protein b0025 [Escherichia coli K12] | 170 | 1.0e-43
|
| paer | PA4561 [gene=ribF] [prot=riboflavin kinase/FAD synthase] [comment=PA4561] | 191 | 7.5e-50
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| FAD_syn | 1 | FAD synthetase | 12-158 | 175.2 | 8e-52
|
| Flavokinase | 1 | Riboflavin kinase | 188-310 | 112.5 | 1.1e-32
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_202497383.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces ...
| 1635 | 0.0e+00 |
| WP_138050551.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces ...
| 1564 | 0.0e+00 |
| WP_119105700.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces ...
| 1560 | 0.0e+00 |
| WP_189327189.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces ...
| 1553 | 0.0e+00 |
| WP_033326867.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces ...
| 1549 | 0.0e+00 |
| WP_055493409.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces ...
| 1548 | 0.0e+00 |
| NGO15004.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces scabi...
| 1547 | 0.0e+00 |
| MPY46013.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces phyll...
| 1544 | 0.0e+00 |
| WP_200395549.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces ...
| 1542 | 0.0e+00 |
| NGO48825.1 | bifunctional riboflavin kinase/FAD synthetase [Streptomyces ureil...
| 1540 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.2260 | Seems to have no N-terminal signal seq.
|