| ID | SAVERM_2548
| Type | CDS
|
| From | 3127969
| nt length | 906
|
| To | 3127064
| aa length | 301
|
| Gene | truB
| pI | 7.72
|
| Definition | putative tRNA pseudouridine synthase
| M.W (Da) | 32111
|
| Note | PF01509: TruB family pseudouridylate synthase (N terminal domain)
| AseI frg. | V
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA modification
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5709 probable tRNA pseudouridine synthase | 535 | 0.0e+00
|
| sgr | SGR_1808 putative tRNA pseudouridine synthase | 499 | 0.0e+00
|
| sam | SAM23877_5430 tRNA pseudouridine synthase B | 542 | 0.0e+00
|
| scab | SCAB25321 putative tRNA pseudouridine synthase | 548 | 0.0e+00
|
| sace | SACE_5919 hypothetical protein | 297 | 1.0e-98
|
| nfar | nfa40530 truB tRNA pseudouridine synthase | 275 | 3.5e-75
|
| mtub | gi|15609930 tRNA pseudouridine synthase B [Mycobacterium tuberculosis H37Rv] | 264 | 6.3e-72
|
| cglu | gi|19553183 COG0130:Pseudouridine synthase [Corynebacterium glutamicum] | 265 | 2.6e-72
|
| bsub | BG10271 truB, ylxN, ymxF; tRNA pseudouridine 55 synthase [EC:4.2.1.70] | 156 | 1.6e-39
|
| ecoli | gi|16131058 tRNA pseudouridine synthase B [Escherichia coli K12] | 142 | 3.6e-35
|
| paer | PA4742 [gene=truB] [prot=tRNA pseudouridine 55 synthase] [comment=PA4742] | 159 | 2.3e-40
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| TruB_N | 1 | TruB family pseudouridylate synthase (N terminal domain) | 31-187 | 158.5 | 1.3e-46
|
| TruB_C | 1 | tRNA Pseudouridine synthase II, C terminal | 243-300 | 64.7 | 4.3e-18
|
| TruB_C_2 | 1 | tRNA pseudouridylate synthase B C-terminal domain | 188-230 | 49.4 | 3.4e-13
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_168530491.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces sp. RPA4-...
| 1381 | 0.0e+00 |
| WP_148010765.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces sp. me109...
| 1379 | 0.0e+00 |
| WP_028802533.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces sp. 142MF...
| 1377 | 0.0e+00 |
| WP_189189077.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces albiflave...
| 1373 | 0.0e+00 |
| WP_190039216.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces fructofer...
| 1370 | 0.0e+00 |
| WP_153288271.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces fagopyri]...
| 1370 | 0.0e+00 |
| WP_168496202.1 | MULTISPECIES: tRNA pseudouridine(55) synthase TruB [Streptom...
| 1367 | 0.0e+00 |
| WP_149825634.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces tailanens...
| 1367 | 0.0e+00 |
| WP_210579014.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces sp. GESEQ...
| 1367 | 0.0e+00 |
| WP_148024164.1 | tRNA pseudouridine(55) synthase TruB [Streptomyces lavendula...
| 1365 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.2488 | Seems to have no N-terminal signal seq.
|