| ID | SAVERM_2561
| Type | CDS
|
| From | 3141613
| nt length | 1158
|
| To | 3140456
| aa length | 385
|
| Gene | ispG1
| pI | 5.06
|
| Definition | putative 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
| M.W (Da) | 40673
|
| Note | gcpE, MEP pathway
| AseI frg. | V
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | specific pathways
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5696 putative 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase | 753 | 0.0e+00
|
| sgr | SGR_1821 putative 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase | 723 | 0.0e+00
|
| sam | SAM23877_5417 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase 2 | 758 | 0.0e+00
|
| scab | SCAB25451 putative 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase | 744 | 0.0e+00
|
| sace | SACE_5992 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase | 633 | 0.0e+00
|
| nfar | nfa41180 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase | 624 | 3.3e-180
|
| mtub | gi|15610005 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Mycobacterium tuberculosis H37Rv] | 593 | 6.1e-171
|
| cglu | gi|19553217 COG0821:Essential bacterial protein, involved in density-dependent regulation of peptidoglycan biosynthesis [Corynebacterium glutamicum] | 579 | 6.4e-167
|
| ecoli | gi|16130440 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli K12] | 314 | 7.1e-87
|
| paer | PA3803 [gene=PA3803] [prot=conserved hypothetical protein] [comment=PA3803] | 338 | 3.7e-94
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| GcpE | 1 | GcpE protein | 22-361 | 453.7 | 3.8e-136
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_105974985.1 | MULTISPECIES: flavodoxin-dependent (E)-4-hydroxy-3-methylbut...
| 1970 | 0.0e+00 |
| WP_165268203.1 | flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphos...
| 1964 | 0.0e+00 |
| WP_194074014.1 | flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphos...
| 1962 | 0.0e+00 |
| WP_055533285.1 | flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphos...
| 1962 | 0.0e+00 |
| WP_037886415.1 | flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphos...
| 1961 | 0.0e+00 |
| WP_094221078.1 | MULTISPECIES: flavodoxin-dependent (E)-4-hydroxy-3-methylbut...
| 1960 | 0.0e+00 |
| WP_063483234.1 | flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphos...
| 1959 | 0.0e+00 |
| WP_053753526.1 | flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphos...
| 1959 | 0.0e+00 |
| WP_164320957.1 | flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphos...
| 1959 | 0.0e+00 |
| WP_196458753.1 | flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphos...
| 1958 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.0901 | Seems to have no N-terminal signal seq.
|