| ID | SAVERM_2748
| Type | CDS
|
| From | 3377715
| nt length | 2193
|
| To | 3375523
| aa length | 730
|
| Gene | ligA
| pI | 4.93
|
| Definition | putative DNA ligase
| M.W (Da) | 80423
|
| Note | PF01653: NAD-dependent DNA ligase adenylation domain
| AseI frg. | J
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | DNA replication
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5494 SC8D9.06 putative DNA ligase | 1219 | 0.0e+00
|
| sgr | SGR_2021 putative DNA ligase | 1244 | 0.0e+00
|
| sam | SAM23877_5244 DNA ligase 1 | 1196 | 0.0e+00
|
| scab | SCAB27251 putative DNA ligase | 1221 | 0.0e+00
|
| sace | SACE_6177 DNA ligase, NAD-dependent | 833 | 0.0e+00
|
| nfar | nfa42650 lig DNA ligase | 799 | 0.0e+00
|
| mtub | gi|15610151 DNA ligase [Mycobacterium tuberculosis H37Rv] | 805 | 0.0e+00
|
| cglu | gi|19552465 COG0272:NAD-dependent DNA ligase (contains BRCT domain type II) [Corynebacterium glutamicum] | 674 | 0.0e+00
|
| bsub | BG12835 ligA, yerG; NAD-dependent DNA ligase [EC:6.5.1.2] | 479 | 3.1e-136
|
| ecoli | gi|16130337 DNA ligase [Escherichia coli K12] | 469 | 2.1e-133
|
| paer | PA1529 [gene=lig] [prot=DNA ligase] [comment=PA1529] | 439 | 5.5e-124
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| DNA_ligase_aden | 1 | NAD-dependent DNA ligase adenylation domain | 15-328 | 409.7 | 6.9e-123
|
| DNA_ligase_OB | 1 | NAD-dependent DNA ligase OB-fold domain | 332-408 | 98.7 | 1.2e-28
|
| HHH_2 | 1 | Helix-hairpin-helix motif | 556-619 | 77.8 | 4.7e-22
|
| BRCT | 1 | BRCA1 C Terminus (BRCT) domain | 640-709 | 48.8 | 6.4e-13
|
| DNA_ligase_ZBD | 1 | NAD-dependent DNA ligase C4 zinc finger domain | 417-445 | 33.0 | 4.1e-08
|
| PTCB-BRCT | 1 | twin BRCT domain | 647-706 | 31.9 | 8.4e-08
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_095852473.1 | MULTISPECIES: NAD-dependent DNA ligase LigA [unclassified St...
| 3485 | 0.0e+00 |
| WP_190173246.1 | NAD-dependent DNA ligase LigA [Streptomyces mirabilis] >dbj
| 3484 | 0.0e+00 |
| WP_060901247.1 | MULTISPECIES: NAD-dependent DNA ligase LigA [unclassified St...
| 3477 | 0.0e+00 |
| WP_181649959.1 | NAD-dependent DNA ligase LigA [Streptomyces sp. WAC00263] >l...
| 3477 | 0.0e+00 |
| WP_143602998.1 | MULTISPECIES: NAD-dependent DNA ligase LigA [unclassified St...
| 3475 | 0.0e+00 |
| WP_217568377.1 | NAD-dependent DNA ligase LigA [Streptomyces sp. GbtcB7]
| 3473 | 0.0e+00 |
| WP_067370880.1 | NAD-dependent DNA ligase LigA [Streptomyces olivochromogenes...
| 3467 | 0.0e+00 |
| WP_075031591.1 | NAD-dependent DNA ligase LigA [Streptomyces mirabilis] >emb
| 3450 | 0.0e+00 |
| NEB27576.1 | NAD-dependent DNA ligase LigA [Streptomyces sp. SID14446]
| 3449 | 0.0e+00 |
| WP_081219567.1 | NAD-dependent DNA ligase LigA [Streptomyces sp. M41(2017)] >...
| 3447 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.3099 | Seems to have no N-terminal signal seq.
|