| ID | SAVERM_2912
| Type | CDS
|
| From | 3644246
| nt length | 1077
|
| To | 3643170
| aa length | 358
|
| Gene | prfA
| pI | 4.8
|
| Definition | putative peptide chain release factor 1
| M.W (Da) | 39503
|
| Note | PF00472: Peptidyl-tRNA hydrolase domain
| AseI frg. | K
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | termination
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO5360 prfA peptide chain release factor | 658 | 0.0e+00
|
| sgr | SGR_2176 putative peptide chain release factor 1 | 682 | 0.0e+00
|
| sam | SAM23877_5105 peptide chain release factor RF-1 | 656 | 0.0e+00
|
| scab | SCAB28891 putative peptide chain release factor 1 | 657 | 0.0e+00
|
| sace | SACE_6292 putative peptide chain release factor 1 | 453 | 4.0e-161
|
| nfar | nfa10540 prfA peptide chain release factor 1 | 447 | 8.5e-127
|
| mtub | gi|15608439 peptide chain release factor 1 [Mycobacterium tuberculosis H37Rv] | 431 | 3.7e-122
|
| cglu | gi|19552424 COG0216:Protein chain release factor A [Corynebacterium glutamicum] | 403 | 7.6e-114
|
| bsub | BG11244 prfA; peptide chain release factor 1 | 349 | 1.2e-97
|
| ecoli | gi|16129174 peptide chain release factor 1 [Escherichia coli K12] | 325 | 3.7e-90
|
| paer | PA4665 [gene=prfA] [prot=peptide chain release factor 1] [comment=PA4665] | 303 | 1.2e-83
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| PCRF | 1 | PCRF domain | 11-206 | 230.9 | 1e-68
|
| RF-1 | 1 | RF-1 domain | 214-323 | 134.1 | 1.9e-39
|
BLAST search result (top 10 hits)
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.3560 | Seems to have no N-terminal signal seq.
|