| ID | SAVERM_3330
| Type | CDS
|
| From | 4145296
| nt length | 876
|
| To | 4144421
| aa length | 291
|
| Gene | birA
| pI | 4.8
|
| Definition | putative biotin apo-protein ligase
| M.W (Da) | 30208
|
| Note | PF03099: Biotin/lipoate A/B protein ligase family
| AseI frg. | E
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of coenzymes & prosthetic groups
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO4927 putative ligase | 434 | 1.0e-155
|
| sgr | SGR_2615 putative biotin apo-protein ligase | 361 | 7.0e-127
|
| sam | SAM23877_4748 putative ligase | 420 | 3.0e-150
|
| scab | SCAB34561 putative biotin apo-protein ligase | 429 | 1.0e-153
|
| sace | SACE_6501 biotin--[acetyl-CoA-carboxylase] ligase | 188 | 2.0e-59
|
| nfar | nfa9950 birA biotin-[acetyl-CoA carboxylase] holoenzyme synthetase/biotin operon repressor | 215 | 4.1e-57
|
| mtub | gi|15610415 biotin--protein ligase [Mycobacterium tuberculosis H37Rv] | 186 | 2.1e-48
|
| cglu | gi|19551939 COG0340:Biotin-(acetyl-CoA carboxylase) ligase [Corynebacterium glutamicum] | 152 | 2.3e-38
|
| bsub | BG11206 birA; transcriptional repressor of the biotin operon / biotin acetyl-CoA-carboxylase synthetase [EC:6.3.4.15] | 121 | 5.5e-29
|
| ecoli | gi|16131807 biotin--protein ligase [Escherichia coli K12] | 100 | 1.5e-22
|
| paer | PA4280 [gene=birA] [prot=BirA bifunctional protein] [comment=PA4280] | 86 | 3.0e-18
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| BPL_C | 1 | Biotin protein ligase C terminal domain | 235-282 | 43.7 | 1.8e-11
|
| BPL_LplA_LipB | 1 | Biotin/lipoate A/B protein ligase family | 45-169 | 40.9 | 1.6e-10
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_210585506.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces sp. GE...
| 1126 | 1.5e-153 |
| WP_216591748.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces brasil...
| 1126 | 1.7e-153 |
| WP_188182129.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces sp. TR...
| 1126 | 1.8e-153 |
| WP_189879078.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces viridi...
| 1123 | 4.7e-153 |
| WP_125514677.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces sp. WA...
| 1121 | 7.8e-153 |
| WP_060890835.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces europa...
| 1122 | 8.1e-153 |
| WP_055528463.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces alboni...
| 1121 | 8.2e-153 |
| WP_138902042.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces albido...
| 1122 | 8.7e-153 |
| WP_228621035.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces sp. JA...
| 1121 | 9.2e-153 |
| WP_097249461.1 | biotin--[acetyl-CoA-carboxylase] ligase [Streptomyces sp. 12...
| 1121 | 9.8e-153 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.3109 | Seems to have no N-terminal signal seq.
|