| ID | SAVERM_3549
| Type | CDS
|
| From | 4394151
| nt length | 3531
|
| To | 4390621
| aa length | 1176
|
| Gene | mfd
| pI | 5.09
|
| Definition | putative transcriptional-repair coupling factor
| M.W (Da) | 128708
|
| Note | PF02559: CarD-like/TRCF domain
| AseI frg. | E
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA elongation
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO3109 putative transcriptional-repair coupling factor | 2171 | 0.0e+00
|
| sgr | SGR_4422 putative transcriptional-repair coupling factor | 2113 | 0.0e+00
|
| sam | SAM23877_3158 Transcription-repair-coupling factor | 2150 | 0.0e+00
|
| scab | SCAB54321 putative transcriptional-repair coupling factor | 2165 | 0.0e+00
|
| sace | SACE_0826 transcription-repair coupling factor | 1410 | 0.0e+00
|
| nfar | nfa48750 mfd transcription-repair coupling factor | 1306 | 0.0e+00
|
| mtub | gi|15608160 PROBABLE TRANSCRIPTION-REPAIR COUPLING FACTOR MFD (TRCF) [Mycobacterium tuberculosis H37Rv] | 1238 | 0.0e+00
|
| cglu | gi|19552188 COG1197:Transcription-repair coupling factor - superfamily II helicase [Corynebacterium glutamicum] | 1144 | 0.0e+00
|
| bsub | BG10118 mfd; transcription-repair coupling factor | 718 | 0.0e+00
|
| ecoli | gi|16129077 transcription-repair ATP-dependent coupling factor [Escherichia coli K12] | 669 | 0.0e+00
|
| paer | PA3002 [gene=mfd] [prot=transcription-repair coupling protein Mfd] [comment=PA3002] | 662 | 0.0e+00
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| CarD_CdnL_TRCF | 1 | CarD-like/TRCF domain | 499-598 | 109.8 | 7.6e-32
|
| TRCF | 1 | TRCF domain | 1032-1114 | 85.6 | 2.1e-24
|
| DEAD | 1 | DEAD/DEAH box helicase | 630-790 | 60.3 | 1.8e-16
|
| Helicase_C | 1 | Helicase conserved C-terminal domain | 833-932 | 58.6 | 6.5e-16
|
| ResIII | 1 | Type III restriction enzyme, res subunit | 629-784 | 25.2 | 1.3e-05
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_131571980.1 | transcription-repair coupling factor [Streptomyces sp. KM273...
| 5501 | 0.0e+00 |
| WP_099501806.1 | MULTISPECIES: transcription-repair coupling factor [Streptom...
| 5498 | 0.0e+00 |
| WP_152869206.1 | transcription-repair coupling factor [Streptomyces acidicola...
| 5483 | 0.0e+00 |
| WP_151477001.1 | transcription-repair coupling factor [Streptomyces albicerus...
| 5479 | 0.0e+00 |
| WP_152775453.1 | transcription-repair coupling factor [Streptomyces spongiae]...
| 5476 | 0.0e+00 |
| WP_062722176.1 | transcription-repair coupling factor [Streptomyces caeruleat...
| 5475 | 0.0e+00 |
| WP_188289861.1 | transcription-repair coupling factor [Streptomyces sp. TRM68...
| 5471 | 0.0e+00 |
| WP_067376188.1 | transcription-repair coupling factor [Streptomyces olivochro...
| 5469 | 0.0e+00 |
| WP_217573967.1 | transcription-repair coupling factor [Streptomyces sp. GbtcB...
| 5466 | 0.0e+00 |
| WP_058924875.1 | transcription-repair coupling factor [Streptomyces sp. CdTB0...
| 5465 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.1461 | Seems to have no N-terminal signal seq.
|