| ID | SAVERM_3966
| Type | CDS
|
| From | 4892763
| nt length | 942
|
| To | 4891822
| aa length | 313
|
| Gene |
| pI | 10.41
|
| Definition | putative tRNA/rRNA methyltransferase
| M.W (Da) | 33000
|
| Note | PF00588: SpoU rRNA Methylase family
| AseI frg. | G1
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA modification
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO4236 putative tRNA/rRNA methyltransferase | 536 | 0.0e+00
|
| sgr | SGR_4020 putative tRNA/rRNA methyltransferase | 497 | 5.0e-180
|
| sam | SAM23877_3464 putative tRNA/rRNA methyltransferase | 538 | 0.0e+00
|
| scab | SCAB50121 putative tRNA/rRNA methyltransferase | 566 | 0.0e+00
|
| sace | SACE_0444 tRNA/rRNA methyltransferase | 345 | 4.0e-120
|
| nfar | nfa4390 tRNA/rRNA methyltransferase | 344 | 5.0e-96
|
| mtub | gi|15610715 POSSIBLE TRNA/RRNA METHYLTRANSFERASE [Mycobacterium tuberculosis H37Rv] | 317 | 5.0e-88
|
| cglu | gi|19553838 COG0566:rRNA methylases [Corynebacterium glutamicum] | 294 | 3.2e-81
|
| bsub | BG10157 yacO; hypothetical tRNA/rRNA methyltransferase yacO [EC:2.1.1.-] | 192 | 2.2e-50
|
| ecoli | gi|16132002 Gm2251 methyltransferase of 23S rRNA [Escherichia coli K12] | 148 | 5.2e-37
|
| paer | PA4936 [gene=PA4936] [prot=probable rRNA methylase] [comment=PA4936] | 147 | 1.6e-36
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| SpoU_methylase | 1 | SpoU rRNA Methylase family | 165-306 | 111.2 | 4.2e-32
|
| SpoU_sub_bind | 1 | RNA 2'-O ribose methyltransferase substrate binding | 72-148 | 64.8 | 6e-18
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_095935146.1 | 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB [Stre...
| 1515 | 0.0e+00 |
| WP_033322303.1 | MULTISPECIES: 23S rRNA (guanosine(2251)-2'-O)-methyltransfer...
| 1515 | 0.0e+00 |
| WP_028805684.1 | 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB [Stre...
| 1510 | 0.0e+00 |
| WP_130441915.1 | 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB [Stre...
| 1509 | 0.0e+00 |
| WP_165339457.1 | 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB [Stre...
| 1507 | 0.0e+00 |
| WP_189186137.1 | 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB [Stre...
| 1502 | 0.0e+00 |
| WP_165270901.1 | 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB [Stre...
| 1500 | 0.0e+00 |
| WP_190170846.1 | MULTISPECIES: 23S rRNA (guanosine(2251)-2'-O)-methyltransfer...
| 1500 | 0.0e+00 |
| WP_009329491.1 | 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB [Stre...
| 1499 | 0.0e+00 |
| WP_208782084.1 | 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB [Stre...
| 1498 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.1150 | Seems to have no N-terminal signal seq.
|