| ID | SAVERM_4206
| Type | CDS
|
| From | 5162486
| nt length | 1677
|
| To | 5160810
| aa length | 558
|
| Gene | fadD9
| pI | 4.94
|
| Definition | putative acyl-CoA synthetase, long-chain fatty acid:CoA ligase
| M.W (Da) | 60622
|
| Note | PF00501: AMP-binding enzyme
| AseI frg. | H
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of lipids
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO4006 putative fatty acid CoA ligase | 993 | 0.0e+00
|
| sgr | SGR_3602 putative acyl-CoA synthetase | 875 | 0.0e+00
|
| sam | SAM23877_3675 Medium-chain-fatty-acid--CoA ligase | 999 | 0.0e+00
|
| scab | SCAB47091 putative acyl-CoA synthetase, long-chain fatty acid:CoA ligase | 960 | 0.0e+00
|
| sace | SACE_6960 putative fatty acid:CoA ligase | 691 | 0.0e+00
|
| nfar | nfa7120 acyl-CoA synthetase | 675 | 0.0e+00
|
| mtub | gi|15608198 acyl-CoA synthase [Mycobacterium tuberculosis H37Rv] | 576 | 1.2e-165
|
| cglu | gi|19552422 COG0318:Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II [Corynebacterium glutamicum] | 470 | 6.5e-134
|
| paer | PA3924 [gene=PA3924] [prot=probable medium-chain acyl-CoA ligase] [comment=PA3924] | 353 | 1.7e-98
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| AMP-binding | 1 | AMP-binding enzyme | 38-452 | 285.5 | 5.1e-85
|
| AMP-binding_C | 1 | AMP-binding enzyme C-terminal domain | 462-538 | 62.0 | 7.3e-17
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_109497062.1 | long-chain fatty acid--CoA ligase [Streptomyces sp. Act143] ...
| 2655 | 0.0e+00 |
| WP_189708915.1 | long-chain fatty acid--CoA ligase [Streptomyces anandii]
| 2648 | 0.0e+00 |
| WP_189869635.1 | long-chain fatty acid--CoA ligase [Streptomyces viridiviolac...
| 2645 | 0.0e+00 |
| WP_075031418.1 | long-chain fatty acid--CoA ligase [Streptomyces mirabilis]
| 2642 | 0.0e+00 |
| WP_189152134.1 | long-chain fatty acid--CoA ligase [Streptomyces lacrimiflumi...
| 2642 | 0.0e+00 |
| WP_188184411.1 | long-chain fatty acid--CoA ligase [Streptomyces sp. TRM S81-...
| 2639 | 0.0e+00 |
| WP_149547846.1 | long-chain fatty acid--CoA ligase [Streptomyces marokkonensi...
| 2639 | 0.0e+00 |
| WP_174479438.1 | MULTISPECIES: long-chain fatty acid--CoA ligase [Streptomyce...
| 2638 | 0.0e+00 |
| WP_199214950.1 | long-chain fatty acid--CoA ligase [Streptomyces sp. BSE7-9] ...
| 2636 | 0.0e+00 |
| QIY96970.1 | long-chain fatty acid--CoA ligase [Streptomyces sp. S1D4-11]
| 2634 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.1617 | Seems to have no N-terminal signal seq.
|