| ID | SAVERM_4299
| Type | CDS
|
| From | 5268401
| nt length | 1443
|
| To | 5266959
| aa length | 480
|
| Gene | pcnA
| pI | 5.31
|
| Definition | putative RNA nucleotidyltransferase
| M.W (Da) | 53875
|
| Note | PF01743: Poly A polymerase family
| AseI frg. | H
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA modification
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO3896 putative RNA nucleotidyltransferase | 886 | 0.0e+00
|
| sgr | SGR_3683 putative RNA nucleotidyltransferase | 883 | 0.0e+00
|
| sam | SAM23877_3760 RNA nucleotidyltransferase | 891 | 0.0e+00
|
| scab | SCAB45991 putative RNA nucleotidyltransferase | 897 | 0.0e+00
|
| sace | SACE_7377 poly(A) polymerase | 627 | 0.0e+00
|
| nfar | nfa56560 tRNA nucleotidyltransferase | 583 | 1.4e-167
|
| mtub | gi|57117168 PROBABLE POLY(A) POLYMERASE PCNA (POLYNUCLEOTIDE ADENYLYLTRANSFERASE) (NTP POLYMERASE) (RNA ADENYLATING ENZYME) (POLY(A) POLYMERASE) [Mycobacterium tuberculosis H37Rv] | 568 | 3.6e-163
|
| cglu | gi|19554274 COG0617:tRNA nucleotidyltransferase/poly(A) polymerase [Corynebacterium glutamicum] | 553 | 8.4e-159
|
| bsub | BG11208 cca, papS, ypjI; tRNA CCA-adding enzyme [EC:2.7.7.25] | 136 | 3.1e-33
|
| ecoli | gi|16130952 tRNA nucleotidyl transferase [Escherichia coli K12] | 117 | 1.3e-27
|
| paer | PA0584 [gene=cca] [prot=tRNA nucleotidyl transferase] [comment=PA0584] | 117 | 2.3e-27
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; bsub, Bacillus subtilis; ecoli, Escherichia coli K-12; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| PolyA_pol | 1 | Poly A polymerase head domain | 46-168 | 113.9 | 5.8e-33
|
| PolyA_pol_RNAbd | 1 | Probable RNA and SrmB- binding site of polymerase A | 195-256 | 72.5 | 1.6e-20
|
| HD | 1 | HD domain | 270-364 | 57.9 | 1.1e-15
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_202498236.1 | CCA tRNA nucleotidyltransferase [Streptomyces sp. SID5469]
| 2448 | 0.0e+00 |
| WP_055632136.1 | CCA tRNA nucleotidyltransferase [Streptomyces griseoruber] >...
| 2405 | 0.0e+00 |
| WP_151477266.1 | CCA tRNA nucleotidyltransferase [Streptomyces albicerus]
| 2395 | 0.0e+00 |
| WP_143627272.1 | MULTISPECIES: CCA tRNA nucleotidyltransferase [unclassified ...
| 2393 | 0.0e+00 |
| WP_099966602.1 | CCA tRNA nucleotidyltransferase [Streptomyces sp. JV178] >gb...
| 2390 | 0.0e+00 |
| WP_097253551.1 | CCA tRNA nucleotidyltransferase [Streptomyces sp. Ag109_G2-1...
| 2390 | 0.0e+00 |
| WP_054236443.1 | MULTISPECIES: CCA tRNA nucleotidyltransferase [unclassified ...
| 2389 | 0.0e+00 |
| WP_143602285.1 | MULTISPECIES: CCA tRNA nucleotidyltransferase [unclassified ...
| 2388 | 0.0e+00 |
| WP_067000438.1 | CCA tRNA nucleotidyltransferase [Streptomyces cellostaticus]...
| 2387 | 0.0e+00 |
| WP_159769987.1 | CCA tRNA nucleotidyltransferase [Streptomyces sp. HM190]
| 2387 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.3729 | Seems to have no N-terminal signal seq.
|