| ID | SAVERM_4378
| Type | CDS
|
| From | 5358524
| nt length | 1389
|
| To | 5359912
| aa length | 462
|
| Gene | bkdH
| pI | 6.2
|
| Definition | putative dihydrolipoamide acyltransferase component
| M.W (Da) | 48745
|
| Note | PF00198: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
| AseI frg. | H
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of lipids
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO3815 putative dihydrolipoamide acyltransferase component | 704 | 0.0e+00
|
| sgr | SGR_3767 putative dihydrolipoamide acyltransferase component | 662 | 0.0e+00
|
| sam | SAM23877_3843 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex | 696 | 0.0e+00
|
| scab | SCAB45161 putative dihydrolipoamide acyltransferase component | 719 | 0.0e+00
|
| sace | SACE_7296 putative dihydrolipoamide acyltransferase component | 430 | 6.0e-149
|
| nfar | nfa10230 branched-chain alpha-keto acid dehydrogenase component | 305 | 7.1e-84
|
| bsub | BG10209 pdhC, aceC; pyruvate dehydrogenase (dihydrolipoamide acetyltransferase E2 subunit) [EC:2.3.1.12] | 281 | 5.6e-77
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; bsub, Bacillus subtilis;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| 2-oxoacid_dh | 1 | 2-oxoacid dehydrogenases acyltransferase (catalytic domain) | 231-459 | 261.9 | 4.3e-78
|
| Biotin_lipoyl | 1 | Biotin-requiring enzyme | 8-79 | 81.0 | 3.8e-23
|
| E3_binding | 1 | e3 binding domain | 165-199 | 52.1 | 5.8e-14
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_097253466.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. Ag109_...
| 1848 | 0.0e+00 |
| WP_220293375.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. MBT84]...
| 1837 | 0.0e+00 |
| WP_149823515.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces tailanensi...
| 1809 | 0.0e+00 |
|
| 1808 | 0.0e+00 |
| WP_223180746.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. TRM S8...
| 1807 | 0.0e+00 |
| WP_215155240.1 | MULTISPECIES: 2-oxo acid dehydrogenase subunit E2 [unclassif...
| 1806 | 0.0e+00 |
| WP_062145658.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces curacoi] >...
| 1806 | 0.0e+00 |
| WP_071378123.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces sp. MUSC 1...
| 1804 | 0.0e+00 |
| WP_189780827.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces capitiform...
| 1799 | 0.0e+00 |
| WP_067018445.1 | 2-oxo acid dehydrogenase subunit E2 [Streptomyces dysideae] ...
| 1795 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.2105 | Seems to have no N-terminal signal seq.
|