| ID | SAVERM_5123
| Type | CDS
|
| From | 6221200
| nt length | 597
|
| To | 6221796
| aa length | 198
|
| Gene | sig45
| pI | 8.94
|
| Definition | putative RNA polymerase ECF-subfamily sigma factor
| M.W (Da) | 22176
|
| Note | similar to SCO2954, PF04542: Sigma-70 region 2
| AseI frg. | B
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | RNA initiation
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO2954 putative RNA polymerase sigma factor | 371 | 7.0e-134
|
| sam | SAM23877_2991 ECF RNA polymerase sigma factor SigL | 340 | 6.0e-122
|
| scab | SCAB55901 putative RNA polymerase sigma factor | 360 | 8.0e-130
|
| sace | SACE_5521 RNA polymerase, sigma-24 subunit, ECF subfamily | 138 | 5.0e-42
|
| nfar | nfa55120 sigma factor | 147 | 8.0e-37
|
| mtub | gi|15607875 RNA polymerase sigma-70 factor [Mycobacterium tuberculosis H37Rv] | 127 | 5.1e-31
|
*coel, Streptomyces coelicolor A3(2); sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| Sigma70_r2 | 1 | Sigma-70 region 2 | 40-108 | 58.3 | 4.4e-16
|
| Sigma70_r4 | 1 | Sigma-70, region 4 | 140-188 | 52.0 | 3.3e-14
|
| Sigma70_r4_2 | 1 | Sigma-70, region 4 | 136-186 | 35.6 | 4.9e-09
|
| Sigma70_ECF | 1 | ECF sigma factor | 92-183 | 26.1 | 6.2e-06
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_030380473.1 | MULTISPECIES: sigma-70 family RNA polymerase sigma factor [u...
| 872 | 9.4e-118 |
| WP_078630833.1 | MULTISPECIES: sigma-70 family RNA polymerase sigma factor [S...
| 869 | 2.3e-117 |
| WP_079040643.1 | sigma-70 family RNA polymerase sigma factor [Streptomyces pr...
| 860 | 5.5e-116 |
|
| 860 | 5.7e-116 |
| WP_079162127.1 | sigma-70 family RNA polymerase sigma factor [Streptomyces no...
| 857 | 2.3e-115 |
| WP_103841015.1 | MULTISPECIES: sigma-70 family RNA polymerase sigma factor [u...
| 855 | 2.9e-115 |
|
| 853 | 4.6e-115 |
| PJM94860.1 | RNA polymerase subunit sigma [Streptomyces sp. CB01373]
| 853 | 5.1e-115 |
| WP_079173430.1 | MULTISPECIES: sigma-70 family RNA polymerase sigma factor [S...
| 853 | 5.7e-115 |
| WP_030348495.1 | MULTISPECIES: sigma-70 family RNA polymerase sigma factor [S...
| 853 | 6.3e-115 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.4517 | Seems to have no N-terminal signal seq.
|