| ID | SAVERM_5601
| Type | CDS
|
| From | 6774328
| nt length | 1722
|
| To | 6776049
| aa length | 573
|
| Gene | leuA2
| pI | 4.61
|
| Definition | putative 2-isopropylmalate synthase
| M.W (Da) | 63564
|
| Note | PF00682: HMGL-like
| AseI frg. | B
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of amino acids & related molecules
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO2528 2-ispoprylmalate synthase | 1066 | 0.0e+00
|
| sgr | SGR_5012 putative 2-isopropylmalate synthase | 1033 | 0.0e+00
|
| sam | SAM23877_2578 2-isopropylmalate synthase | 1075 | 0.0e+00
|
| scab | SCAB61851 putative 2-isopropylmalate synthase | 1066 | 0.0e+00
|
| sace | SACE_0274 2-isopropylmalate synthase | 762 | 0.0e+00
|
| nfar | nfa3080 leuA 2-isopropylmalate synthase | 743 | 0.0e+00
|
| mtub | gi|15610846 2-isopropylmalate synthase [Mycobacterium tuberculosis H37Rv] | 692 | 0.0e+00
|
| cglu | gi|19551500 COG0119:Isopropylmalate/homocitrate/citramalate synthases [Corynebacterium glutamicum] | 650 | 0.0e+00
|
| paer | PA3792 [gene=leuA] [prot=2-isopropylmalate synthase] [comment=PA3792] | 606 | 2.0e-174
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; cglu, Corynebacterium glutamicum; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| HMGL-like | 1 | HMGL-like | 37-318 | 246.9 | 2.3e-73
|
| LeuA_dimer | 1 | LeuA allosteric (dimerisation) domain | 439-571 | 127.3 | 3.2e-37
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_168491384.1 | MULTISPECIES: 2-isopropylmalate synthase [Streptomyces] >gb
| 2863 | 0.0e+00 |
| QIY95811.1 | 2-isopropylmalate synthase [Streptomyces sp. S1D4-11]
| 2860 | 0.0e+00 |
| WP_097284668.1 | MULTISPECIES: 2-isopropylmalate synthase [unclassified Strep...
| 2859 | 0.0e+00 |
| WP_054235463.1 | MULTISPECIES: 2-isopropylmalate synthase [unclassified Strep...
| 2855 | 0.0e+00 |
| WP_060896475.1 | 2-isopropylmalate synthase [Streptomyces sp. WAC00263] >lcl
| 2854 | 0.0e+00 |
| WP_099922729.1 | MULTISPECIES: 2-isopropylmalate synthase [Streptomyces] >dbj...
| 2853 | 0.0e+00 |
| WP_153290699.1 | 2-isopropylmalate synthase [Streptomyces fagopyri] >gb
| 2853 | 0.0e+00 |
| WP_075026795.1 | 2-isopropylmalate synthase [Streptomyces mirabilis] >emb
| 2853 | 0.0e+00 |
| WP_095854754.1 | MULTISPECIES: 2-isopropylmalate synthase [unclassified Strep...
| 2852 | 0.0e+00 |
| WP_067369872.1 | 2-isopropylmalate synthase [Streptomyces olivochromogenes] >...
| 2852 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| cytoplasm | 0.4413 | Seems to have no N-terminal signal seq.
|