| ID | SAVERM_6407
| Type | CDS
|
| From | 7683001
| nt length | 1602
|
| To | 7681400
| aa length | 533
|
| Gene | cobJ
| pI | 4.67
|
| Definition | putative precorrin methylase
| M.W (Da) | 55021
|
| Note | CbiG/H-fusion; cobalamin biosynthesis, PF01890: CbiG
| AseI frg. | S
|
| SignalP v.4.0 | Non-secretory protein | plot
| Category | metabolism of coenzymes & prosthetic groups
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| coel | SCO1857 putative bifunctional protein (CbiGH) | 877 | 0.0e+00
|
| sgr | SGR_3822 putative precorrin methylase | 171 | 8.0e-48
|
| sam | SAM23877_1929 Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase | 890 | 0.0e+00
|
| scab | SCAB9801 putative precorrin methylase | 165 | 9.0e-46
|
| sace | SACE_5960 putative precorrin methylase | 222 | 2.0e-67
|
| nfar | nfa31520 cobIJ precorrin 2 methyltransferase/precorrin 3B methyltransferase | 184 | 1.7e-47
|
| mtub | gi|15609203 Probable bifunctional protein, CobI-CobJ fusion protein: S-adenosyl-L-methionine-precorrin-2 methyl transferase + precorrin-3 methylase [Mycobacterium tuberculosis H37Rv] | 177 | 1.5e-45
|
| paer | PA2903 [gene=cobJ] [prot=precorrin-3 methylase CobJ] [comment=PA2903] | 248 | 1.3e-66
|
*coel, Streptomyces coelicolor A3(2); sgr, Streptomyces griseus IFO13350; sam, Streptomyces ambofaciens ATCC 23877; scab, Streptomyces scabies 87.22; sace, Saccharopolyspora erythraea NRRL2338; nfar, Nocardia farcinica IFM 10152; mtub, Mycobacterium tuberculosis H37Rv; paer, Pseudomonas aeruginosa;
Pfam search result
| Model | No. | Description | Position (aa) | Score | E-value
|
| TP_methylase | 1 | Tetrapyrrole (Corrin/Porphyrin) Methylases | 290-495 | 131.3 | 4.3e-38
|
| CbiG_N | 1 | Cobalamin synthesis G N-terminal | 9-89 | 121.9 | 7.3e-36
|
| CbiG_C | 1 | Cobalamin synthesis G C-terminus | 164-282 | 117.6 | 4e-34
|
BLAST search result (top 10 hits)
| ID | Description | Score | E-value
|
| WP_237529686.1 | precorrin-3B C(17)-methyltransferase [Streptomyces sp. SID54...
| 2331 | 0.0e+00 |
| KOT38252.1 | Precorrin methylase [Streptomyces caelestis] >gb
| 2109 | 0.0e+00 |
| WP_030833800.1 | MULTISPECIES: precorrin-3B C(17)-methyltransferase [Streptom...
| 2109 | 0.0e+00 |
| WP_086603558.1 | precorrin-3B C(17)-methyltransferase [Streptomyces swartberg...
| 2098 | 0.0e+00 |
| WP_190095895.1 | precorrin-3B C(17)-methyltransferase [Streptomyces griseofla...
| 2096 | 0.0e+00 |
| WP_142151122.1 | precorrin-3B C(17)-methyltransferase [Streptomyces sp. SLBN-...
| 2096 | 0.0e+00 |
| WP_193506380.1 | precorrin-3B C(17)-methyltransferase [Streptomyces bellus] >...
| 2092 | 0.0e+00 |
| WP_234490753.1 | precorrin-3B C(17)-methyltransferase [Streptomyces sp. MBT33...
| 2091 | 0.0e+00 |
| WP_055613081.1 | precorrin-3B C(17)-methyltransferase [Streptomyces phaeochro...
| 2091 | 0.0e+00 |
| WP_055491596.1 | precorrin-3B C(17)-methyltransferase [Streptomyces sp. TP-A0...
| 2090 | 0.0e+00 |
PSORT result
| Localization | Certainty | PSORT said
|
| membrane | 0.2826 | Seems to have no N-terminal signal seq.
|