Molecule: Escherichi coli MG1655 chromosome Escherichi coli BW25113 chromosome Escherichi coli S17-1 chromosome Escherichi coli DH5alpha chromosome Escherichi coli DH10B chromosome Escherichi coli BL21(DE3) chromosome Escherichi coli RR1 chromosome From 0 1 2 3 4 5 6 7 8 9 . 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 Mbp Sequence from: to: Span: 1,000 bp 2,500 bp 5,000 bp 10,000 bp 25,000 bp 50,000 bp 75,000 bp 100,000 bp 150,000 bp 200,000 bp 250,000 bp 500,000 bp 1,000,000 bp Enzyme 1: AatII(GACGTC) AarI(CACCTGCnnnnnnnn) Acc36I(ACCTGCnnnnnnnn) AccII(CGCG) AflII(CTTAAG) AgeI(ACCGGT) AluI(AGCT) ApaI(GGGCCC) ApaLI(GTGCAC) ApoI(rAATTy) AscI(GGCGCGCC) AseI(ATTAAT) AvrII(CCTAGG) BamHI(GGATCC) BbsI(GAAGACnnnnnn) BbvCI(CCTCAGG) BciVI(GTATCCnnnnnn) BclI(TGATCA) BfaI(CTAG) BfiI(ACTGGGnnnnn) BglII(AGATCT) BmgBI(CACGTC) BlpI(GCTNAGC) Bpu10I(CCTNAGC) BsaI(GGTCTCnnnnn) BseYI(CCCAGC) BsiWI(CGTACG) BsmAI(GTCTCnnnnn) BsmBI(CGTCTCnnnnn) BspEI(TCCGGA) BspHI(TCATGA) BspLU11I(ACATGT) BspQI(GCTCTTCnnnn) BsrBI(CCGCTC) BsrGI(TGTACA) BssHII(GCGCGC) BssSI(CACGAG) Bst1107I(GTATAC) BstBI(TTCGAA) BstYI(rGATCy) Bsu36I(CCTNAGG) ClaI(ATCGAT) DraI(TTTAAA) EagI(CGGCCG) EarI(CTCTTCnnnnnn Eco47III(AGCGCT) EcoRI(GAATTC) EcoRV(GATATC) FseI(GGCCGGCC) FspI(TGCGCA) HaeIII(GGCC) HhaI(GCGC) HindIII(AAGCTT) HpaI(GTTAAC) HpaII(CCGG) IPpoI(CTCTCTTAAGGTAGC) KpnI(GGTACC) MboI(GATC) MfeI(CAATTG) MluI(ACGCGT) MscI(TGGCCA) MseI(TTAA) NarI(GGCGCC) NcoI(CCATGG) NdeI(CATATG) NgoMI(GCCGGC) NheI(GCTAGC) NlaIII(CATG) NotI(GCGGCCGC) NruI(TCGCGA) NsiI(ATGCAT) PacI(TTAATTAA) PmeI(GTTTAAAC) PmlI(CACGTG) PsiI(TTATAA) Psp1406I(AACGTT) PstI(CTGCAG) PvuI(CGATCG) PvuII(CAGCTG) RsaI(GTAC) SacI(GAGCTC) SacII(CCGCGG) SalI(GTCGAC) SapI(GCTCTTCnnnn) ScaI(AGTACT) SgfI(GCGATCGC) SmaI(CCCGGG) SnaBI(TACGTA) SpeI(ACTAGT) SphI(GCATGC) SrfI(GCCCGGGC) Sse8387I(CCTGCAGG) SspI(AATATT) StuI(AGGCCT) SwaI(ATTTAAAT) TaqI(TCGA) Tsp509I(AATT) TthHB8I(TCGA) XbaI(TCTAGA) XhoI(CTCGAG) ZraI(GACGTC) CTAG-family GATC-family Enzyme 2: nothing AatII(GACGTC) AarI(CACCTGCnnnnnnnn) Acc36I(ACCTGCnnnnnnnn) AccII(CGCG) AflII(CTTAAG) AgeI(ACCGGT) AluI(AGCT) ApaI(GGGCCC) ApaLI(GTGCAC) ApoI(rAATTy) AscI(GGCGCGCC) AseI(ATTAAT) AvrII(CCTAGG) BamHI(GGATCC) BbsI(GAAGACnnnnnn) BbvCI(CCTCAGG) BciVI(GTATCCnnnnnn) BclI(TGATCA) BfaI(CTAG) BfiI(ACTGGGnnnnn) BglII(AGATCT) BmgBI(CACGTC) BlpI(GCTNAGC) Bpu10I(CCTNAGC) BsaI(GGTCTCnnnnn) BseYI(CCCAGC) BsiWI(CGTACG) BsmAI(GTCTCnnnnn) BsmBI(CGTCTCnnnnn) BspEI(TCCGGA) BspHI(TCATGA) BspLU11I(ACATGT) BspQI(GCTCTTCnnnn) BsrBI(CCGCTC) BsrGI(TGTACA) BssHII(GCGCGC) BssSI(CACGAG) Bst1107I(GTATAC) BstBI(TTCGAA) BstYI(rGATCy) Bsu36I(CCTNAGG) ClaI(ATCGAT) DraI(TTTAAA) EagI(CGGCCG) EarI(CTCTTCnnnnnn Eco47III(AGCGCT) EcoRI(GAATTC) EcoRV(GATATC) FseI(GGCCGGCC) FspI(TGCGCA) HaeIII(GGCC) HhaI(GCGC) HindIII(AAGCTT) HpaI(GTTAAC) HpaII(CCGG) IPpoI(CTCTCTTAAGGTAGC) KpnI(GGTACC) MboI(GATC) MfeI(CAATTG) MluI(ACGCGT) MscI(TGGCCA) MseI(TTAA) NarI(GGCGCC) NcoI(CCATGG) NdeI(CATATG) NgoMI(GCCGGC) NheI(GCTAGC) NlaIII(CATG) NotI(GCGGCCGC) NruI(TCGCGA) NsiI(ATGCAT) PacI(TTAATTAA) PmeI(GTTTAAAC) PmlI(CACGTG) PsiI(TTATAA) Psp1406I(AACGTT) PstI(CTGCAG) PvuI(CGATCG) PvuII(CAGCTG) RsaI(GTAC) SacI(GAGCTC) SacII(CCGCGG) SalI(GTCGAC) SapI(GCTCTTCnnnn) ScaI(AGTACT) SgfI(GCGATCGC) SmaI(CCCGGG) SnaBI(TACGTA) SpeI(ACTAGT) SphI(GCATGC) SrfI(GCCCGGGC) Sse8387I(CCTGCAGG) SspI(AATATT) StuI(AGGCCT) SwaI(ATTTAAAT) TaqI(TCGA) Tsp509I(AATT) TthHB8I(TCGA) XbaI(TCTAGA) XhoI(CTCGAG) ZraI(GACGTC) Enzyme 3: nothing AatII(GACGTC) AarI(CACCTGCnnnnnnnn) Acc36I(ACCTGCnnnnnnnn) AccII(CGCG) AflII(CTTAAG) AgeI(ACCGGT) AluI(AGCT) ApaI(GGGCCC) ApaLI(GTGCAC) ApoI(rAATTy) AscI(GGCGCGCC) AseI(ATTAAT) AvrII(CCTAGG) BamHI(GGATCC) BbsI(GAAGACnnnnnn) BbvCI(CCTCAGG) BciVI(GTATCCnnnnnn) BclI(TGATCA) BfaI(CTAG) BfiI(ACTGGGnnnnn) BglII(AGATCT) BmgBI(CACGTC) BlpI(GCTNAGC) Bpu10I(CCTNAGC) BsaI(GGTCTCnnnnn) BseYI(CCCAGC) BsiWI(CGTACG) BsmAI(GTCTCnnnnn) BsmBI(CGTCTCnnnnn) BspEI(TCCGGA) BspHI(TCATGA) BspLU11I(ACATGT) BspQI(GCTCTTCnnnn) BsrBI(CCGCTC) BsrGI(TGTACA) BssHII(GCGCGC) BssSI(CACGAG) Bst1107I(GTATAC) BstBI(TTCGAA) BstYI(rGATCy) Bsu36I(CCTNAGG) ClaI(ATCGAT) DraI(TTTAAA) EagI(CGGCCG) EarI(CTCTTCnnnnnn Eco47III(AGCGCT) EcoRI(GAATTC) EcoRV(GATATC) FseI(GGCCGGCC) FspI(TGCGCA) HaeIII(GGCC) HhaI(GCGC) HindIII(AAGCTT) HpaI(GTTAAC) HpaII(CCGG) IPpoI(CTCTCTTAAGGTAGC) KpnI(GGTACC) MboI(GATC) MfeI(CAATTG) MluI(ACGCGT) MscI(TGGCCA) MseI(TTAA) NarI(GGCGCC) NcoI(CCATGG) NdeI(CATATG) NgoMI(GCCGGC) NheI(GCTAGC) NlaIII(CATG) NotI(GCGGCCGC) NruI(TCGCGA) NsiI(ATGCAT) PacI(TTAATTAA) PmeI(GTTTAAAC) PmlI(CACGTG) PsiI(TTATAA) Psp1406I(AACGTT) PstI(CTGCAG) PvuI(CGATCG) PvuII(CAGCTG) RsaI(GTAC) SacI(GAGCTC) SacII(CCGCGG) SalI(GTCGAC) SapI(GCTCTTCnnnn) ScaI(AGTACT) SgfI(GCGATCGC) SmaI(CCCGGG) SnaBI(TACGTA) SpeI(ACTAGT) SphI(GCATGC) SrfI(GCCCGGGC) Sse8387I(CCTGCAGG) SspI(AATATT) StuI(AGGCCT) SwaI(ATTTAAAT) TaqI(TCGA) Tsp509I(AATT) TthHB8I(TCGA) XbaI(TCTAGA) XhoI(CTCGAG) ZraI(GACGTC)
